3D cell microscopy enables high-precision, dynamic three-dimensional observation of living cells through molecular labeling technology12, high-speed optical imaging system38 and intelligent algorithm-driven three-dimensional reconstruction46.
Multi-angle imaging technology
Through multi-angle light sources or detector arrays, the three-dimensional information of the sample is obtained from different directions, image shadows and artifacts are reduced, and imaging clarity is improved.
Super-resolution microscopy technology
Stimulated emission depletion (STED): Breaking through the optical diffraction limit, achieving nanometer-level resolution, and clearly presenting subcellular structures.
Single-molecule localization microscopy (SMLM) and structured illumination microscopy (SIM): Improve resolution through special optical processing.
Light sheet illumination technology
Use extremely thin light sheets to illuminate the sample in parallel, improve Z-axis resolution, and support three-dimensional tomography.
High-speed three-dimensional scanning technology
Capture images at a speed of hundreds to thousands of frames per second to record cell dynamic activities in real time.
Three-dimensional reconstruction algorithm
Combined with computer-aided technology, multi-angle images are integrated to generate a three-dimensional structural model to intuitively display cell morphology and spatial distribution.
Fluorescence Lifetime Imaging (FLIM)
Enhance imaging contrast and functional analysis capabilities by detecting the luminescence decay characteristics of fluorescent molecules.
Digital Adaptive Optics Technology
Dynamically correct optical distortion and improve the imaging signal-to-noise ratio under weak light conditions.
Scanning Light Field Imaging Principle
Combined with virtual scanning algorithms, high-speed, large field of view (centimeter-level) mesoscopic three-dimensional imaging is achieved.
Confocal Laser Scanning Technology
Eliminate background interference through the conjugate focusing principle and improve the quality of optical cross-sectional imaging.
Non-destructive Observation Technology
Use non-invasive imaging methods to avoid damage to living cells and ensure data reliability.
High-resolution Objective System
With advanced optical design, analyze the internal structure of cells (such as mitochondria, endoplasmic reticulum, etc.) at the nanometer level.
Summary
The key technologies of 3D cell microscopy cover multiple aspects such as optical design, data processing, and system architecture. Through multidisciplinary integration, high-precision, dynamic three-dimensional observation of living cells is achieved, providing a powerful tool for biomedical research and clinical diagnosis.
