FIS4 biological cell wavefront sensor 400-1100nm

FIS4 biological cell wavefront sensor 400-1100nm

The Bojiong FIS4 biological cell wavefront sensor 400-1100nm is a label-free quantitative cell imager specifically designed for the biomedical field. Based on four-wave lateral shearing interferometry technology, it enables non-invasive, stain-free, high-resolution, real-time 3D imaging and quantitative analysis of living cells. With an ultra-high spatial resolution of 512×512 and an axial sensitivity of better than 2 nm, the  FIS4 biological cell wavefront sensor 400-1100nm clearly captures cell morphology, dry weight, dynamic volume changes, and the phase distribution of subcellular structures.

Bojiong FIS4 biological cell wavefront sensor 400-1100nm Introduction

The Bojiong FIS4 biological cell wavefront sensor 400-1100nm is a high-precision optical measurement instrument designed for demanding scientific research and industrial inspection scenarios. It utilizes an advanced common-path interferometer design combined with a real-time wavefront reconstruction algorithm to achieve precise measurement without phase shifting, and boasts excellent vibration resistance and environmental adaptability. The FIS4 biological cell wavefront sensor 400-1100nm complies with ISO 9001 quality management system requirements and is certified by the China Institute of Metrology (NIM). It comes with a one-year warranty, ensuring the reliability and traceability of measurement data. The FIS4 biological cell wavefront sensor 400-1100nm plug-and-play nature and simplified maintenance allow users to quickly deploy and continuously achieve stable, high-performance performance.

Bojiong FIS4 biological cell wavefront sensor 400-1100nm Parameter (Specification)

Bojiong FIS4 biological cell wavefront sensor 400-1100nm Feature And Application

Since 2006, after 17 years of dedicated research and development by Professor Yang Yongying's team at Zhejiang University, the FIS4 series wavefront sensors, featuring innovative common-path optical design and real-time wavefront reconstruction algorithms, has been officially launched. This product breaks through the technical bottlenecks of traditional wavefront detection and sets a new benchmark for high-precision optical measurement.

·Nanometer-level detection sensitivity: Axial resolution reaches 2nm, enabling precise quantification of cell mass, thickness, and morphological changes.

·Plug-and-play integration: A single-optical common-path interferometry design eliminates the need for a reference optical path and is directly compatible with most microscope systems.

·Real-time quantitative analysis: Supports 10fps full-resolution phase imaging and outputs multi-dimensional data such as cell dry weight, volume, and morphology in real time.

·Compact and portable design: The sensor module is only the size of a fist, eliminating the need for a vibration isolation platform and adapting to various experimental environments.

This biological cell wavefront sensor deeply integrates advanced wavefront sensing technology with life science research, providing researchers with a simpler, more accurate, and more efficient label-free cell analysis solution. It supports near-infrared light and LED white light wide-spectrum illumination. Its imaging process does not require labeling or staining, achieving in vivo 3D imaging with an imaging frame rate of up to 24fps. It is an ideal tool for label-free 3D living cell detection, biological tissue detection, germ cell detection, and transmissive micro-nanostructure detection.

FIS4 biological cell wavefront sensor 400-1100nm Application

Bojiong FIS4 biological cell wavefront sensor 400-1100nm Details

The FIS4 biological cell wavefront sensor 400-1100nm , centered around innovative patented randomly coded four-wave diffraction technology, achieves wavefront self-interference with a single beam, placing the interference process in the back-end image plane. This technology maintains exceptional environmental vibration resistance and ultra-high operational stability, even without vibration isolation, enabling nanometer-level measurement accuracy. Compared to traditional microlens array Hartmann sensors, the FIS4 FIS4 biological cell wavefront sensor 400-1100nm offers significant advantages: higher phase point acquisition density, a wider spectral adaptability range, a larger dynamic measurement range, and superior overall cost-performance. With its revolutionary technical architecture, this sensor provides a more reliable and economical advanced solution for the field of optical inspection.

图 1 基于随机编码混合光栅的四波前横向剪切干涉相位成像原理

Fig.1.Phase imaging principle based on four-wave lateral shearing interference using randomly coded hybrid grating (REHG)

图 2.四波前横向剪切干涉图的最小二乘波前重构计算方法

Fig.2.Least square wavefront reconstruction from four-wave lateral shearing interferogram

The FIS4 wavefront sensor, with its compact design, exceptional environmental robustness, high temporal resolution, and excellent compatibility with existing microscope systems, has become a powerful and widely used high-precision measurement tool in research and industry. Initially used for basic inspection tasks in optical workshops, including optical component quality assessment, laser beam performance analysis, and adaptive optics system tuning, its applications have now expanded to cutting-edge fields such as biomedical imaging, nanoparticle tracking, metasurface structure analysis, and even temperature gradient visualization.

The FIS4 wavefront sensor's compact design allows for seamless integration into a variety of microscopy platforms. Its robust vibration tolerance ensures stable interferometric measurement accuracy even in challenging environments. The device supports rapid single-exposure measurement, enabling accurate capture of transient dynamic processes. In biomedical research, the FIS4 wavefront sensor has enabled label-free, high-resolution, real-time imaging of a variety of living cells, including COS-7, HT1080, RPE, CHO, HEK, and neuronal cells, providing powerful technical support for life science research. In terms of imaging capabilities, FIS4 boasts exceptional phase-delay imaging, enabling high-contrast images of anisotropic biological tissues and subcellular structures, such as collagen fiber alignment and cytoskeletal networks. This technology has now been extended to include X-ray, mid-wave infrared (MWIR), and long-wave infrared (LWIR) wavelengths, demonstrating strong interdisciplinary application potential. Furthermore,the FIS4 wavefront sensor has been widely applied in emerging materials science research, such as metasurface manipulation and two-dimensional material characterization, fully demonstrating its multifaceted application value in modern optics and materials science.